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Analysis of Transforming Growth Factor ß Family Cleavage Products Secreted Into the Blastocoele of Xenopus laevis Embryos
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Analysis of Transforming Growth Factor ß Family Cleavage Products Secreted Into the Blastocoele of Xenopus laevis Embryos

Analysis of Transforming Growth Factor ß Family Cleavage Products Secreted Into the Blastocoele of Xenopus laevis Embryos

DOI:
10.3791/62782-v

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06:57 min

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July 21, 2021

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,
1Department of Neurobiology,University of Utah, 2Departments of Neurobiology and Department of Internal Medicine, Division of Hematology and Hematologic Malignancies,University of Utah, School of Medicine

Capitoli

  • 00:04Conclusion
  • 00:50Blastocoele Extraction and Analysis of Tgf β Cleavage Products
  • 04:49Results: Analysis of Cleaved BMP Ligands in Xenopus laevis Blastocoele Fluid
  • 06:08Conclusion

Summary

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When Transforming Growth Factor ß family precursor proteins are ectopically expressed in Xenopus laevis embryos, they dimerize, get cleaved and are secreted into the blastocoele, which begins at the late blastula to early gastrula stage. We describe a method for aspirating cleavage products from the blastocoele cavity for immunoblot analysis.

Tags

TGF-betaXenopus LaevisEmbryosBlastocele FluidProteolytic CleavageAspirateMicroinjectorImmunoblotPrecursor ProteinsCleavage Products

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