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自动、高通量检测细菌对宿主细胞的粘附
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Automated, High-Throughput Detection of Bacterial Adherence to Host Cells
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自动、高通量检测细菌对宿主细胞的粘附

DOI:
10.3791/62764-v

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07:21 min

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September 17, 2021

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, , , ,
1Department of Microbial Pathogenesis and Immunology,Texas A&M Health Science Center, 2College of Veterinary Medicine,Texas A&M University

Chapters

  • 00:04Introduction
  • 00:48Staining
  • 02:20Bacterial Adherence Assay
  • 04:01Automated Fluorescence Imaging, Processing, and Analysis
  • 05:24Results: Analysis of Host Cell-Adhered Bacteria
  • 06:46Conclusion

Summary

자동 번역

자동 번역

使用高通量荧光标记成像以及自动统计分析方法，基于表型粘附性检测宿主 - 细菌病原体相互作用，可以快速评估细菌与宿主细胞的潜在相互作用。

Tags

Bacterial AdherenceHigh-throughput DetectionHost CellsAutomatedTime-savingReproducibleMultiplicity Of InfectionBacterial SuspensionStainingBacterial Adherence AssayA549 CellsPBSFormaldehyde

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