Dergi
/
İmmünoloji ve Enfeksiyon
/
Automated, High-Throughput Detection of Bacterial Adherence to Host Cells
JoVE Journal
İmmünoloji ve Enfeksiyon
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal İmmünoloji ve Enfeksiyon
Automated, High-Throughput Detection of Bacterial Adherence to Host Cells

Automated, High-Throughput Detection of Bacterial Adherence to Host Cells

DOI:
10.3791/62764-v

•

07:21 min

•

September 17, 2021

•

, , , ,
1Department of Microbial Pathogenesis and Immunology,Texas A&M Health Science Center, 2College of Veterinary Medicine,Texas A&M University

Bölümler

  • 00:04Introduction
  • 00:48Staining
  • 02:20Bacterial Adherence Assay
  • 04:01Automated Fluorescence Imaging, Processing, and Analysis
  • 05:24Results: Analysis of Host Cell-Adhered Bacteria
  • 06:46Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Detection of host-bacterial pathogen interactions based on phenotypic adherence using high-throughput fluorescence labeling imaging along with automated statistical analysis methods enables rapid evaluation of potential bacterial interactions with host cells.

Etiketler

Bacterial AdherenceHigh-throughput DetectionHost CellsAutomatedTime-savingReproducibleMultiplicity Of InfectionBacterial SuspensionStainingBacterial Adherence AssayA549 CellsPBSFormaldehyde

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

High-throughput Detection Method for Influenza Virus

Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells

Automated Separation of C. elegans Variably Colonized by a Bacterial Pathogen

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells

Implementation of a Permeable Membrane Insert-based Infection System to Study the Effects of Secreted Bacterial Toxins on Mammalian Host Cells

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.

High-throughput Measurement of Plasma Membrane Resealing Efficiency in Mammalian Cells

Using a Bacterial Pathogen to Probe for Cellular and Organismic-level Host Responses

A High-throughput Shigella-specific Bactericidal Assay

Read Article