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Measuring the pH, Redox Chemistries, and Degradative Capacity of Macropinosomes using Dual-Fluorophore Ratiometric Microscopy
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Measuring the pH, Redox Chemistries, and Degradative Capacity of Macropinosomes using Dual-Fluorophore Ratiometric Microscopy

Measuring the pH, Redox Chemistries, and Degradative Capacity of Macropinosomes using Dual-Fluorophore Ratiometric Microscopy

2,260 Views

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07:31 min

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August 19, 2021

DOI:

10.3791/62733-v

DOI:
10.3791/62733-v

•

07:31 min
August 19, 2021

•

2 Views
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1Department of Biochemistry & Microbiology, Faculty of Science,University of Victoria, 2Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Science,University of Calgary, 3Calvin, Joan and Phoebe Snyder Institute for Chronic Diseases,University of Calgary

Summary

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We describe protocols for measuring pH, oxidative events, and protein digestion in individual macropinosomes in live cells. An emphasis is placed on dual-fluorophore ratiometric microscopy and the advantages it offers over population-based techniques.

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Wilkinson, L., Canton, J. Measuring the pH, Redox Chemistries, and Degradative Capacity of Macropinosomes using Dual-Fluorophore Ratiometric Microscopy. J. Vis. Exp. (174), e62733, doi:10.3791/62733 (2021).

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