Dergi
/
İmmünoloji ve Enfeksiyon
/
Measuring the pH, Redox Chemistries, and Degradative Capacity of Macropinosomes using Dual-Fluorophore Ratiometric Microscopy
JoVE Journal
İmmünoloji ve Enfeksiyon
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal İmmünoloji ve Enfeksiyon
Measuring the pH, Redox Chemistries, and Degradative Capacity of Macropinosomes using Dual-Fluorophore Ratiometric Microscopy

Measuring the pH, Redox Chemistries, and Degradative Capacity of Macropinosomes using Dual-Fluorophore Ratiometric Microscopy

2,260 Views

•

07:31 min

•

August 19, 2021

DOI:

10.3791/62733-v

DOI:
10.3791/62733-v

•

07:31 min
August 19, 2021

•

2 Views
,
1Department of Biochemistry & Microbiology, Faculty of Science,University of Victoria, 2Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Science,University of Calgary, 3Calvin, Joan and Phoebe Snyder Institute for Chronic Diseases,University of Calgary

Özet

Automatically generated

We describe protocols for measuring pH, oxidative events, and protein digestion in individual macropinosomes in live cells. An emphasis is placed on dual-fluorophore ratiometric microscopy and the advantages it offers over population-based techniques.

Explore More Videos

MacropinosomesPHRedoxDegradationDual-fluorophoreRatiometric MicroscopyFluoresceinTMRHBSSPotassium Rich SolutionNigericinRAW264.7 Cells

Read Article

Cite this Article

Wilkinson, L., Canton, J. Measuring the pH, Redox Chemistries, and Degradative Capacity of Macropinosomes using Dual-Fluorophore Ratiometric Microscopy. J. Vis. Exp. (174), e62733, doi:10.3791/62733 (2021).

Download .ris file

Copy

Share Video

.

Copy