/
/
Production of Adeno-Associated Virus Vectors in Cell Stacks for Preclinical Studies in Large Animal Models
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Production of Adeno-Associated Virus Vectors in Cell Stacks for Preclinical Studies in Large Animal Models
5,369 Views
•
07:21 min
•
June 30, 2021
DOI:
Summary
Automatically generated
Here we provide a detailed procedure for large-scale production of research-grade AAV vectors using adherent HEK 293 cells grown in cell stacks and affinity chromatography purification. This protocol consistently yields >1 x 1013 vector genomes/mL, providing vector quantities appropriate for large animal studies.
Read Article
Cite this Article
Rghei, A. D., Stevens, B. A. Y., Thomas, S. P., Yates, J. G. E., McLeod, B. M., Karimi, K., Susta, L., Bridle, B. W., Wootton, S. K. Production of Adeno-Associated Virus Vectors in Cell Stacks for Preclinical Studies in Large Animal Models. J. Vis. Exp. (172), e62727, doi:10.3791/62727 (2021).
Copy