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Production of Adeno-Associated Virus Vectors in Cell Stacks for Preclinical Studies in Large Animal Models
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June 30, 2021
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Here we provide a detailed procedure for large-scale production of research-grade AAV vectors using adherent HEK 293 cells grown in cell stacks and affinity chromatography purification. This protocol consistently yields >1 x 1013 vector genomes/mL, providing vector quantities appropriate for large animal studies.
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Rghei, A. D., Stevens, B. A. Y., Thomas, S. P., Yates, J. G. E., McLeod, B. M., Karimi, K., Susta, L., Bridle, B. W., Wootton, S. K. Production of Adeno-Associated Virus Vectors in Cell Stacks for Preclinical Studies in Large Animal Models. J. Vis. Exp. (172), e62727, doi:10.3791/62727 (2021).
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