Journal
/
면역학 및 감염병학
/
从人类胚胎干细胞中引导肝细胞样细胞诱导的有效方法
JoVE 신문
면역학 및 감염병학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 면역학 및 감염병학
An Efficient Method for Directed Hepatocyte-Like Cell Induction from Human Embryonic Stem Cells
Please note that all translations are automatically generated. Click here for the English version.

从人类胚胎干细胞中引导肝细胞样细胞诱导的有效方法

DOI:
10.3791/62654-v

•

08:05 min

•

May 06, 2021

•

, , , ,
1Stem Cell Research Center,Shantou University Medical College, 2The Center for Reproductive Medicine,Shantou University Medical College, 3Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology,Shantou University Medical College

Chapters

  • 00:04Introduction
  • 00:45Stem Cell Maintenance
  • 02:28Stem Cell Passage and Differentiation and Definitive Endoderm Formation
  • 03:46Differentiation of Hepatic Progenitor Cells and Hepatocyte Differentiation
  • 05:12Results: Directed Hepatocyte-like Cell Induction from Human Embryonic Stem Cells
  • 07:14Conclusion

Summary

자동 번역

자동 번역

本手稿描述了人类胚胎干细胞（hESC）分化成功能性肝细胞样细胞（HLCs）的详细协议，在hESC分化过程中连续补充Activin A和CHIR99021，以最终内分泌体（DE）。

Tags

Human Embryonic Stem CellsHepatocyte-like CellsDifferentiationDefinitive EndodermFOXA2SOX17GATA4CXCR4FOXA1MatrigelMTESRActivin ACHIR99021Cell CultureDisease ModelingDrug Screening

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

高效转染树突状细胞的无细胞的成熟优化的协议

HLA - IG基于人工抗原提呈细胞可高效体外扩展人类的CTL

定向分化诱导性多潜能干细胞向T淋巴细胞

杀手人工抗原提呈细胞（KaAPC）为有效<em>在体外</em>人抗原特异性T细胞耗竭

对T细胞推导<em>在体外</em>从小鼠胚胎干细胞

一个<em>体外</em>急性脑片的少突胶质细胞定向T细胞攻击模式

类器官的模型传染病：人类的文化与小鼠胃组织体与幽门螺杆菌的显微注射

从人类腭扁桃体B和T淋巴细胞有效隔离协议

小鼠树突状细胞亚群的分离的高效和高产的方法

从小鼠在原代肝细胞培养物的炎性反应的诱导

Read Article