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An Efficient Method for Directed Hepatocyte-Like Cell Induction from Human Embryonic Stem Cells
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Immunologie et infection
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Journal JoVE Immunologie et infection
An Efficient Method for Directed Hepatocyte-Like Cell Induction from Human Embryonic Stem Cells

An Efficient Method for Directed Hepatocyte-Like Cell Induction from Human Embryonic Stem Cells

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08:05 min

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May 06, 2021

DOI:

10.3791/62654-v

DOI:
10.3791/62654-v

•

08:05 min
May 06, 2021

•

3 Views
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1Stem Cell Research Center,Shantou University Medical College, 2The Center for Reproductive Medicine,Shantou University Medical College, 3Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology,Shantou University Medical College

Summary

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This manuscript describes a detailed protocol for differentiation of human embryonic stem cells (hESCs) into functional hepatocyte-like cells (HLCs) by continuously supplementing Activin A and CHIR99021 during hESC differentiation into definitive endoderm (DE).

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Zhou, Q., Xie, X., Zhong, Z., Sun, P., Zhou, X. An Efficient Method for Directed Hepatocyte-Like Cell Induction from Human Embryonic Stem Cells. J. Vis. Exp. (171), e62654, doi:10.3791/62654 (2021).

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