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1Radiation Biophysics Division, Nuclear Medicine Department,iThemba LABS, 2Department of Biotechnology,University of the Western Cape, 3Department of Medical Biosciences,University of the Western Cape, 4Division of Medical Physiology, Department of Medicine and Health Sciences,Stellenbosch University, 5Biodosimetry Service, Instituto de Investigaciones Biológicas Clemente Estable and Academic Unit on Radiation Protection, Faculty of Medicine,University of the Republic, 6MetaSystems Hard & Software GmbH
Chapters
- 00:00Introduction
- 00:44Preparation of Samples
- 01:26Sample Irradiations
- 01:35Slide Preparation
- 02:17Fixation and Immunofluorescence γ-H2AX Staining
- 03:44Automated Metafer Scanning and Scoring
- 07:29Results
- 08:03Conclusion
This protocol presents the slide preparation and automated scoring of the γ-H2AX foci assay on peripheral blood lymphocytes. To illustrate the method and sensitivity of the assay, isolated lymphocytes were irradiated in vitro. This automated method of DNA DSB detection is useful for fast and high-throughput biological dosimetry applications.
