Journal
/
공학
/
Surgery and Sample Processing for Correlative Imaging of the Murine Pulmonary Valve
JoVE 신문
공학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 공학
Surgery and Sample Processing for Correlative Imaging of the Murine Pulmonary Valve

Surgery and Sample Processing for Correlative Imaging of the Murine Pulmonary Valve

2,417 Views

•

07:34 min

•

August 05, 2021

DOI:

10.3791/62581-v

DOI:
10.3791/62581-v

•

07:34 min
August 05, 2021

•

1 Views
, , , , , , ,
1Center for Electron Microscopy and Analysis, The Department of Materials Science and Engineering,Ohio State University, 2Center for Regenerative Medicine,Nationwide Children’s Hospital, 3Thermo Fisher Scientific

Summary

Automatically generated

Here, we describe a correlative workflow for the excision, pressurization, fixation, and imaging of the murine pulmonary valve to determine the gross conformation and local extracellular matrix structures.

Explore More Videos

Murine Pulmonary ValveCorrelative ImagingStructure-functionControlled FixationHigh-resolution ImagingMouse DissectionThoracotomyPulmonary Artery PressurizationPrimary Fixative Solution

Read Article

Cite this Article

Liu, Y., Lee, Y., Yi, T., Wu, K., Bouchet-Marquis, C., Chan, H., Breuer, C. K., McComb, D. W. Surgery and Sample Processing for Correlative Imaging of the Murine Pulmonary Valve. J. Vis. Exp. (174), e62581, doi:10.3791/62581 (2021).

Download .ris file

Copy

Share Video

.

Copy