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Surgery and Sample Processing for Correlative Imaging of the Murine Pulmonary Valve
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JoVE Journal Ingenieurwesen
Surgery and Sample Processing for Correlative Imaging of the Murine Pulmonary Valve

Surgery and Sample Processing for Correlative Imaging of the Murine Pulmonary Valve

2,417 Views

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07:34 min

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August 05, 2021

DOI:

10.3791/62581-v

DOI:
10.3791/62581-v

•

07:34 min
August 05, 2021

•

1 Views
, , , , , , ,
1Center for Electron Microscopy and Analysis, The Department of Materials Science and Engineering,Ohio State University, 2Center for Regenerative Medicine,Nationwide Children’s Hospital, 3Thermo Fisher Scientific

Summary

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Here, we describe a correlative workflow for the excision, pressurization, fixation, and imaging of the murine pulmonary valve to determine the gross conformation and local extracellular matrix structures.

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Murine Pulmonary ValveCorrelative ImagingStructure-functionControlled FixationHigh-resolution ImagingMouse DissectionThoracotomyPulmonary Artery PressurizationPrimary Fixative Solution

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Cite this Article

Liu, Y., Lee, Y., Yi, T., Wu, K., Bouchet-Marquis, C., Chan, H., Breuer, C. K., McComb, D. W. Surgery and Sample Processing for Correlative Imaging of the Murine Pulmonary Valve. J. Vis. Exp. (174), e62581, doi:10.3791/62581 (2021).

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