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Genomweite Analyse der Histonmodifikationsverteilung mit der Chromatin-Immunpräzipitationssequenzierungsmethode in Magnaporthe Oryzae
Chapters
- 00:04Introduction
- 00:39Preparation of Protoplasts from M. oryzae
- 01:48In Vivo Crosslinking and Sonication
- 03:22IP of Crosslinked Protein/DNA
- 04:23Collecting and Rinsing the IP Products
- 05:04Elution and Reverse Crosslinking of Protein/DNA Complexes
- 05:56Purification and Recovery of DNA
- 06:51DNA Repair and Solexa Library Construction
- 08:23Results: Chromatin Immunoprecipitation Sequencing Method for Genome-wide Histone Modification Analysi
- 08:53Conclusion
