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Journal / Immunology and Infection / 基因敲击由CRISPR/Cas9和细胞排序在巨噬细胞和T细胞线…
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Immunology and Infection
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JoVE Journal Immunology and Infection
基因敲击由CRISPR/Cas9和细胞排序在巨噬细胞和T细胞线
 
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基因敲击由CRISPR/Cas9和细胞排序在巨噬细胞和T细胞线

Article DOI: 10.3791/62328-v • 11:32 min • November 13th, 2021
November 13th, 2021 •
1, 1, 1, 1, 1, 1, 1, 1, 2, 1
1The Laboratory of Genetic Regulators in the Immune System, School of Laboratory Medicine, Xinxiang Medical University, 2Centre d’Immunologie de Marseille-Luminy, Aix Marseille Université

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该协议使用荧光记者和细胞分拣来简化巨噬细胞和T细胞系的敲击实验。两个质粒用于这些简化的敲击实验，即CRISPR/Cas9-和DsRed2表达质粒和同源重组供体质粒表达EBFP2，这是永久整合在 Rosa26 点在免疫细胞。

Tags

免疫学和感染， 第 177 期， 敲门， CRISPR/Cas9， 罗莎26， 巨噬细胞， T 细胞， hACE2
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