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用 crispr/cas9 基因编辑生成重组禽流感病毒载体
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing
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用 crispr/cas9 基因编辑生成重组禽流感病毒载体

12,364 Views

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12:21 min

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January 07, 2019

DOI:

10.3791/58193-v

DOI:
10.3791/58193-v

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12:21 min
January 07, 2019

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1The Pirbright Institute & UK-China Centre of Excellence for Research on Avian Diseases, 2Binzhou Animal Science and Veterinary Medicine Academy & UK-China Centre of Excellence for Research on Avian Diseases

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CRISPR/Cas9Recombinant Avian HerpesvirusViral AntigensHVT GenomeRecombinant VaccinesGFP Expression CassetteCre-LoxP SystemCell SortingChick Embryo FibroblastsTransfectionHVT Virus StockPlaque Forming UnitsTrypsin-EDTACell SuspensionCell DensityCell SorterGFP-expressing Cells

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Tang, N., Zhang, Y., Pedrera, M., Chang, P., Baigent, S., Moffat, K., Shen, Z., Nair, V., Yao, Y. Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing. J. Vis. Exp. (143), e58193, doi:10.3791/58193 (2019).

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