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Stereocilia Bundle Imaging with Nanoscale Resolution in Live Mammalian Auditory Hair Cells
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Stereocilia Bundle Imaging with Nanoscale Resolution in Live Mammalian Auditory Hair Cells

Stereocilia Bundle Imaging with Nanoscale Resolution in Live Mammalian Auditory Hair Cells

2,928 Views

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06:47 min

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January 21, 2021

DOI:

10.3791/62104-v

DOI:
10.3791/62104-v

•

06:47 min
January 21, 2021

•

1 Views
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1Department of Physiology, College of Medicine,University of Kentucky, 2Universidad Nacional de Colombia

Summary

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Here we present a protocol for the Hopping Probe Ion Conductance Microscopy (HPICM), a non-contact scanning probe technique that allows nanoscale imaging of stereocilia bundles in live auditory hair cells.

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StereociliaHair CellsNanoscale ResolutionLive Cell ImagingHPICMNanopipettesCalibration StandardZ ApproachSurface Topography

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Cite this Article

Galeano-Naranjo, C., Veléz-Ortega, A. C., Frolenkov, G. I. Stereocilia Bundle Imaging with Nanoscale Resolution in Live Mammalian Auditory Hair Cells. J. Vis. Exp. (167), e62104, doi:10.3791/62104 (2021).

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