Here we present a protocol for the Hopping Probe Ion Conductance Microscopy (HPICM), a non-contact scanning probe technique that allows nanoscale imaging of stereocilia bundles in live auditory hair cells.
Galeano-Naranjo, C., Veléz-Ortega, A. C., Frolenkov, G. I. Stereocilia Bundle Imaging with Nanoscale Resolution in Live Mammalian Auditory Hair Cells. J. Vis. Exp. (167), e62104, doi:10.3791/62104 (2021).