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使用多柱板适配器的经济性和多功能性高通量蛋白质净化系统
JoVE 신문
생화학
Author Produced
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JoVE 신문 생화학
An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter
DOI:

10:08 min

May 21, 2021

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Chapters

  • 00:00Introduction
  • 00:58Ni-NTA Chromatography- Purification of 24 Different Proteins: Preparing the Purification Set-up
  • 03:00Ni-NTA Chromatography- Purification of 24 Different Proteins: Loading, Washing and Eluting
  • 04:47Ion Exchange- Simultaneous Purification of 6 Different Proteins: Preparing the Purification Set-up
  • 06:15Ion Exchange- Simultaneous Purification of 6 Different Proteins: Loading, Washing and Eluting
  • 08:25Results: Representative UV Spectroscopy and SDS PAGE Analyses

Summary

자동 번역

多柱板适配器允许色谱柱与多井收集板接口,用于平行亲和力或离子交换纯化,提供经济的高通量蛋白质纯化方法。它可以通过负担得起的仪器在重力或真空下产生毫克蛋白质。

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