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An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter

An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter

DOI:
10.3791/62075-v

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10:08 min

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May 21, 2021

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, , , , ,
1School of Life Sciences,University of Liverpool, 2Health Sciences Center,Texas Tech University

Chapters

  • 00:00Introduction
  • 00:58Ni-NTA Chromatography- Purification of 24 Different Proteins: Preparing the Purification Set-up
  • 03:00Ni-NTA Chromatography- Purification of 24 Different Proteins: Loading, Washing and Eluting
  • 04:47Ion Exchange- Simultaneous Purification of 6 Different Proteins: Preparing the Purification Set-up
  • 06:15Ion Exchange- Simultaneous Purification of 6 Different Proteins: Loading, Washing and Eluting
  • 08:25Results: Representative UV Spectroscopy and SDS PAGE Analyses

Summary

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A multi-column plate adapter allows chromatography columns to be interfaced with multi-well collection plates for parallel affinity or ion exchange purification providing an economical high throughput protein purification method. It can be used under gravity or vacuum yielding milligram quantities of protein via affordable instrumentation.

Tags

Protein PurificationHigh-throughputMulti-column Plate AdapterMCPANickel Affinity ChromatographyIon Exchange ChromatographyParallel PurificationEconomicalVersatileFunctional ProteomicsBiophysical TechniquesVacuumGravityAutomation

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