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Isolating and Imaging Live, Intact Pacemaker Regions of Mouse Renal Pelvis by Vibratome Sectioning
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Isolating and Imaging Live, Intact Pacemaker Regions of Mouse Renal Pelvis by Vibratome Sectioning

Isolating and Imaging Live, Intact Pacemaker Regions of Mouse Renal Pelvis by Vibratome Sectioning

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07:38 min

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April 30, 2021

DOI:

10.3791/62040-v

DOI:
10.3791/62040-v

•

07:38 min
April 30, 2021

•

3 Views
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1Department of Physiology & Cell Biology,University of Nevada, Reno School of Medicine, 2Department of Physiology & Membrane Biology,University of California School of Medicine, 3Department of Life & Health Sciences,Dundalk Institute of Technology

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The goal of this protocol is to isolate intact pacemaker regions of the mouse renal pelvis using vibratome sectioning. These sections can then be used for in situ Ca2+ imaging to elucidate Ca2+ transient properties of pacemaker cells and other interstitial cells in vibratome slices.

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Grainger, N., Sanders, K. M., Drumm, B. T. Isolating and Imaging Live, Intact Pacemaker Regions of Mouse Renal Pelvis by Vibratome Sectioning. J. Vis. Exp. (170), e62040, doi:10.3791/62040 (2021).

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