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Isolating and Imaging Live, Intact Pacemaker Regions of Mouse Renal Pelvis by Vibratome Sectioning
JoVE Journal
Biologie
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JoVE Journal Biologie
Isolating and Imaging Live, Intact Pacemaker Regions of Mouse Renal Pelvis by Vibratome Sectioning

Isolating and Imaging Live, Intact Pacemaker Regions of Mouse Renal Pelvis by Vibratome Sectioning

2,967 Views

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07:38 min

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April 30, 2021

DOI:

10.3791/62040-v

DOI:
10.3791/62040-v

•

07:38 min
April 30, 2021

•

3 Views
, ,
1Department of Physiology & Cell Biology,University of Nevada, Reno School of Medicine, 2Department of Physiology & Membrane Biology,University of California School of Medicine, 3Department of Life & Health Sciences,Dundalk Institute of Technology

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The goal of this protocol is to isolate intact pacemaker regions of the mouse renal pelvis using vibratome sectioning. These sections can then be used for in situ Ca2+ imaging to elucidate Ca2+ transient properties of pacemaker cells and other interstitial cells in vibratome slices.

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Pacemaker RegionsRenal PelvisVibratome SectioningIntact PreparationSmooth MuscleKidneyUreterCalcium Ion ImagingDissectionRazor BladeKRB Solution

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Cite this Article

Grainger, N., Sanders, K. M., Drumm, B. T. Isolating and Imaging Live, Intact Pacemaker Regions of Mouse Renal Pelvis by Vibratome Sectioning. J. Vis. Exp. (170), e62040, doi:10.3791/62040 (2021).

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