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Labelling and Visualization of Mitochondrial Genome Expression Products in Baker’s Yeast Saccharomyces cerevisiae
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Labelling and Visualization of Mitochondrial Genome Expression Products in Baker’s Yeast Saccharomyces cerevisiae

Labelling and Visualization of Mitochondrial Genome Expression Products in Baker’s Yeast Saccharomyces cerevisiae

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08:33 min

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April 11, 2021

DOI:

10.3791/62020-v

DOI:
10.3791/62020-v

•

08:33 min
April 11, 2021

•

2 Views
, , , , ,
1Department of Biology,M.V. Lomonosov Moscow State University, 2Institute of Functional Genomics,M.V. Lomonosov Moscow State University, 3National Research Centre “Kurchatov Institute”

Summary

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Baker’s yeast mitochondrial genome encodes eight polypeptides. The goal of the current protocol is to label all of them and subsequently visualize them as separate bands.

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Mitochondrial GenomeMitochondrial Protein BiosynthesisRadioactive LabelingGel ElectrophoresisSaccharomyces CerevisiaeYeastTranslationCycloheximideS35 MethionineBorymycin

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Cite this Article

Chicherin, I. V., Levitskii, S. A., Baleva, M. V., Krasheninnikov, I. A., Patrushev, M. V., Kamenski, P. A. Labelling and Visualization of Mitochondrial Genome Expression Products in Baker's Yeast Saccharomyces cerevisiae. J. Vis. Exp. (170), e62020, doi:10.3791/62020 (2021).

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