ジャーナル
/
生化学
/
Labelling and Visualization of Mitochondrial Genome Expression Products in Baker’s Yeast Saccharomyces cerevisiae
JoVE Journal
生化学
Author Produced
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 生化学
Labelling and Visualization of Mitochondrial Genome Expression Products in Baker’s Yeast Saccharomyces cerevisiae

Labelling and Visualization of Mitochondrial Genome Expression Products in Baker’s Yeast Saccharomyces cerevisiae

4,229 Views

•

08:33 min

•

April 11, 2021

DOI:

10.3791/62020-v

DOI:
10.3791/62020-v

•

08:33 min
April 11, 2021

•

2 Views
, , , , ,
1生物学科,M.V. Lomonosov Moscow State University, 2Institute of Functional Genomics,M.V. Lomonosov Moscow State University, 3National Research Centre “Kurchatov Institute”

概要

Automatically generated

Baker’s yeast mitochondrial genome encodes eight polypeptides. The goal of the current protocol is to label all of them and subsequently visualize them as separate bands.

Explore More Videos

Mitochondrial GenomeMitochondrial Protein BiosynthesisRadioactive LabelingGel ElectrophoresisSaccharomyces CerevisiaeYeastTranslationCycloheximideS35 MethionineBorymycin

Read Article

Cite this Article

Chicherin, I. V., Levitskii, S. A., Baleva, M. V., Krasheninnikov, I. A., Patrushev, M. V., Kamenski, P. A. Labelling and Visualization of Mitochondrial Genome Expression Products in Baker's Yeast Saccharomyces cerevisiae. J. Vis. Exp. (170), e62020, doi:10.3791/62020 (2021).

Download .ris file

Copy

Share Video

.

Copy