Evaluating the Impact of Hydraulic Fracturing on Streams using Microbial Molecular Signatures

Jeremy  R. Chen See; Olivia Wright; Lavinia V. Unverdorben; Nathan Heibeck; Stephen M. Techtmann; Terry C. Hazen; Regina Lamendella

doi:10.3791/61904

Journal

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환경과학

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Evaluating the Impact of Hydraulic Fracturing on Streams using Microbial Molecular Signatures

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환경과학

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Evaluating the Impact of Hydraulic Fracturing on Streams using Microbial Molecular Signatures

2,874 Views

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09:11 min

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April 04, 2021

DOI:

10.3791/61904-v

DOI:

10.3791/61904-v

•

09:11 min

April 04, 2021

•

5 Views

Jeremy R. Chen See^1,2, Olivia Wright¹, Lavinia V. Unverdorben^1,2, Nathan Heibeck¹, Stephen M. Techtmann³, Terry C. Hazen^4,5, Regina Lamendella^1,2

¹Department of Biology,Juniata College, ²Wright Labs, LLC, ³Department of Biological Sciences,Michigan Technological University, ⁴Biosciences Division,Oak Ridge National Laboratory, ⁵Department of Civil and Environmental Engineering,University of Tennessee

Summary

Here, we present a protocol to investigate the impacts of hydraulic fracturing on nearby streams by analyzing their water and sediment microbial communities.

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Hydraulic Fracturing Streams Microbial Molecular Signatures Sediment Samples Nucleic Acid Extraction 16S DNA Analysis Metatranscriptomics RNA Analysis Filter Collection Polyethersulfone Filter DNA/RNA Preservative

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Evaluating the Impact of Hydraulic Fracturing on Streams using Microbial Molecular Signatures

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