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Evaluating the Impact of Hydraulic Fracturing on Streams using Microbial Molecular Signatures
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환경과학
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Evaluating the Impact of Hydraulic Fracturing on Streams using Microbial Molecular Signatures

Evaluating the Impact of Hydraulic Fracturing on Streams using Microbial Molecular Signatures

DOI:
10.3791/61904-v

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09:11 min

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April 04, 2021

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, , , , , ,
1Department of Biology,Juniata College, 2Wright Labs, LLC, 3Department of Biological Sciences,Michigan Technological University, 4Biosciences Division,Oak Ridge National Laboratory, 5Department of Civil and Environmental Engineering,University of Tennessee

Chapters

  • 00:05Introduction
  • 00:42Sediment Sample Collection
  • 01:40Filter Collection
  • 03:02Nucleic Acid Extraction and Quantification
  • 05:17DNA 16S rRNA Library Creation
  • 05:55DNA 16S rRNA Library Purification
  • 06:46Results: Representative Hydraulic Fracturing Stream Microbial Molecular Signature Analyses
  • 08:23Conclusion

Summary

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자동 번역

Here, we present a protocol to investigate the impacts of hydraulic fracturing on nearby streams by analyzing their water and sediment microbial communities.

Tags

Hydraulic FracturingStreamsMicrobialMolecular SignaturesSediment SamplesNucleic Acid Extraction16S DNA AnalysisMetatranscriptomics RNA AnalysisFilter CollectionPolyethersulfone FilterDNA/RNA Preservative

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