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Evaluating the Impact of Hydraulic Fracturing on Streams using Microbial Molecular Signatures
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JoVE Journal Ambiente
Evaluating the Impact of Hydraulic Fracturing on Streams using Microbial Molecular Signatures

Evaluating the Impact of Hydraulic Fracturing on Streams using Microbial Molecular Signatures

2,874 Views

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09:11 min

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April 04, 2021

DOI:

10.3791/61904-v

DOI:
10.3791/61904-v

•

09:11 min
April 04, 2021

•

5 Views
, , , , , ,
1Department of Biology,Juniata College, 2Wright Labs, LLC, 3Department of Biological Sciences,Michigan Technological University, 4Biosciences Division,Oak Ridge National Laboratory, 5Department of Civil and Environmental Engineering,University of Tennessee

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Here, we present a protocol to investigate the impacts of hydraulic fracturing on nearby streams by analyzing their water and sediment microbial communities.

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Hydraulic FracturingStreamsMicrobialMolecular SignaturesSediment SamplesNucleic Acid Extraction16S DNA AnalysisMetatranscriptomics RNA AnalysisFilter CollectionPolyethersulfone FilterDNA/RNA Preservative

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Cite this Article

Chen See, J. R., Wright, O., Unverdorben, L. V., Heibeck, N., Techtmann, S. M., Hazen, T. C., Lamendella, R. Evaluating the Impact of Hydraulic Fracturing on Streams using Microbial Molecular Signatures. J. Vis. Exp. (170), e61904, doi:10.3791/61904 (2021).

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