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Journal / Biochemistry / 从脂质绑定蛋白和过敏原中去除和替换内源性利根…
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Biochemistry

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JoVE Journal Biochemistry
从脂质绑定蛋白和过敏原中去除和替换内源性利根
 
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从脂质绑定蛋白和过敏原中去除和替换内源性利根

Article DOI: 10.3791/61780-v • 09:09 min • February 24th, 2021
February 24th, 2021 •
1, 1, 1
1Nuclear Magnetic Resonance Group, National Institute of Environmental Health Sciences

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该协议描述了从过敏原中去除内源性脂质，并通过逆相HPLC与热退化用用户指定的配体替换它们。 31P-NMR 和圆形二分法允许快速确认配体去除/装载，并恢复原生过敏原结构。

Tags

生物化学，第168期，过敏原，生物化学，生物物理学，脂肪酸结合蛋白，脂肪酸，脂质，蛋白质结合，蛋白质稳定性
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