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Visualiseren van de Calcitonine Gen-Gerelateerde Peptide Immunoreactieve Innervation van de Rat Cranial Dura Mater met Immunofluorescentie en Neural Tracing
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Visualizing the Calcitonin Gene-Related Peptide Immunoreactive Innervation of the Rat Cranial Dura Mater with Immunofluorescence and Neural Tracing
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Visualiseren van de Calcitonine Gen-Gerelateerde Peptide Immunoreactieve Innervation van de Rat Cranial Dura Mater met Immunofluorescentie en Neural Tracing

3,688 Views

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07:28 min

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January 06, 2021

DOI:

10.3791/61742-v

DOI:
10.3791/61742-v

•

07:28 min
January 06, 2021

•

9 Views
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1Institute of Acupuncture and Moxibustion,China Academy of Chinese Medical Sciences

Summary

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Hier presenteren we een protocol om ruimtelijke correlatie van calcitonine gen-gerelateerde peptide (CGRP)-immunoreactieve zenuwvezels en bloedvaten in de schedel dura mater te visualiseren met behulp van immunofluorescentie en fluorescerende histochemie met respectievelijk CGRP en falloïdine. Bovendien was de oorsprong van deze zenuwvezels retrograde getraceerd met een fluorescerende neurale tracer.

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Cite this Article

Wang, J., Xu, D., Cui, J., She, C., Wang, H., Wu, S., Zou, L., Zhang, J., Bai, W. Visualizing the Calcitonin Gene-Related Peptide Immunoreactive Innervation of the Rat Cranial Dura Mater with Immunofluorescence and Neural Tracing. J. Vis. Exp. (167), e61742, doi:10.3791/61742 (2021).

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