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JoVE Journal Neuroscience
Visualizing the Calcitonin Gene-Related Peptide Immunoreactive Innervation of the Rat Cranial Dura Mater with Immunofluorescence and Neural Tracing
 

Visualizing the Calcitonin Gene-Related Peptide Immunoreactive Innervation of the Rat Cranial Dura Mater with Immunofluorescence and Neural Tracing

Article DOI: 10.3791/61742-v • 07:28 min • January 6th, 2021
January 6th, 2021 •
*1, *1, 1, 1, 1, 1, 1, 1, 1
1Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences
* These authors contributed equally

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Here we present a protocol to visualize spatial correlation of calcitonin gene-related peptide (CGRP)-immunoreactive nerve fibers and blood vessels in the cranial dura mater using immunofluorescence and fluorescent histochemistry with CGRP and phalloidin, respectively. In addition, the origin of these nerve fibers was retrograde traced with a fluorescent neural tracer.

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Calcitonin Gene-Related Peptide Immunoreactive Innervation Rat Cranial Dura Mater Immunofluorescence Neural Tracing Neurovascular Structure Physiological Condition Pathological Condition Transparent Whole Mount Dura Mater 3D View Transcardial Perfusion Paraformaldehyde Phosphate Buffer Head Skin Removal Skull Opening Dura Mater Exposure Dissect Cranial Dura Mater Blocking Solution Mouse Anti-CGRP Antibody Donkey Anti-mouse Alexa Fluor 488 Secondary Antibody Phalloidin 568 Normal Donkey Serum Triton X-100
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