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Visualizing the Calcitonin Gene-Related Peptide Immunoreactive Innervation of the Rat Cranial Dura Mater with Immunofluorescence and Neural Tracing
JoVE Journal
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JoVE Journal Nörobilim
Visualizing the Calcitonin Gene-Related Peptide Immunoreactive Innervation of the Rat Cranial Dura Mater with Immunofluorescence and Neural Tracing

Visualizing the Calcitonin Gene-Related Peptide Immunoreactive Innervation of the Rat Cranial Dura Mater with Immunofluorescence and Neural Tracing

DOI:
10.3791/61742-v

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07:28 min

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January 06, 2021

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, , , , , , , ,
1Institute of Acupuncture and Moxibustion,China Academy of Chinese Medical Sciences

Bölümler

  • 00:04Introduction
  • 00:45Innervation of Rat Cranial Dura Mater
  • 02:34Retrograde Tracing Study with Fluorogold
  • 05:20Results: Neurovascular Structure of the Cranial Dura Mater and Retrograde-labeled Neurons in the TG and DRGs
  • 07:02Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Here we present a protocol to visualize spatial correlation of calcitonin gene-related peptide (CGRP)-immunoreactive nerve fibers and blood vessels in the cranial dura mater using immunofluorescence and fluorescent histochemistry with CGRP and phalloidin, respectively. In addition, the origin of these nerve fibers was retrograde traced with a fluorescent neural tracer.

Etiketler

CGRPDura MaterImmunofluorescenceNeural TracingCranial NervesBlood VesselsRatConfocal MicroscopyWhole MountImmunohistochemistryFluorogold

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