Journal
/
생화학
/
Ultra-High-Speed Western Blot using Immunoreaction Enhancing Technology
JoVE 신문
생화학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생화학
Ultra-High-Speed Western Blot using Immunoreaction Enhancing Technology

Ultra-High-Speed Western Blot using Immunoreaction Enhancing Technology

5,627 Views

•

05:59 min

•

September 26, 2020

DOI:

10.3791/61657-v

DOI:
10.3791/61657-v

•

05:59 min
September 26, 2020

•

2 Views
, , , , ,
1Laboratory of Developmental Neurobiology, Cell and Developmental Biology Center, National Heart, Lung, and Blood Institute,National Institutes of Health, 2United Graduate School of Drug Discovery and Medical Information Sciences,Gifu University

Summary

Automatically generated

An ultra-high-speed western blotting technique is developed by improving the kinetics of antigen-antibody binding through cyclic draining and replenishing (CDR) technology in conjunction with an immunoreaction enhancing agent.

Explore More Videos

Ultra-high-speed Western BlotImmunoreaction Enhancing TechnologyPVDF MembranePrimary AntibodySecondary AntibodyChemiluminescent SubstrateSalad SpinnerCyclic Draining And ReplenishingBiomedical ResearchTime-saving

Read Article

Cite this Article

Higashi, S. L., Yagyu, K., Nagase, H., Pearson, C. S., Geller, H. M., Katagiri, Y. Ultra-High-Speed Western Blot using Immunoreaction Enhancing Technology. J. Vis. Exp. (163), e61657, doi:10.3791/61657 (2020).

Download .ris file

Copy

Share Video

.

Copy