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Ultra-High-Speed Western Blot using Immunoreaction Enhancing Technology
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JoVE Journal Bioquímica
Ultra-High-Speed Western Blot using Immunoreaction Enhancing Technology

Ultra-High-Speed Western Blot using Immunoreaction Enhancing Technology

5,627 Views

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05:59 min

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September 26, 2020

DOI:

10.3791/61657-v

DOI:
10.3791/61657-v

•

05:59 min
September 26, 2020

•

2 Views
, , , , ,
1Laboratory of Developmental Neurobiology, Cell and Developmental Biology Center, National Heart, Lung, and Blood Institute,National Institutes of Health, 2United Graduate School of Drug Discovery and Medical Information Sciences,Gifu University

Summary

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An ultra-high-speed western blotting technique is developed by improving the kinetics of antigen-antibody binding through cyclic draining and replenishing (CDR) technology in conjunction with an immunoreaction enhancing agent.

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Cite this Article

Higashi, S. L., Yagyu, K., Nagase, H., Pearson, C. S., Geller, H. M., Katagiri, Y. Ultra-High-Speed Western Blot using Immunoreaction Enhancing Technology. J. Vis. Exp. (163), e61657, doi:10.3791/61657 (2020).

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