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高分解能融解解析を用いた CALR 遺伝子の遺伝的変異体検出
Chapters
- 00:04Introduction
- 01:02Fluorescence-based Quantitative Real-time PCR (qPCR) and Post-qPCR Analysis by HRM
- 05:32Agarose Gel Electrophoresis
- 06:50Results: Detection of the Genetic Variants in the CALR Gene
- 08:21Conclusion
Tags
Genetic Variant DetectionCALR GeneHigh Resolution Melting AnalysisMyeloproliferative DisordersEssential ThrombocythemiaPrimary MyelofibrosisQPCR HRM Master MixNegative ControlsPositive ControlsNo Template Control (NTC)Optical Adhesive FilmDissociation AnalysisAmplification PlotFluorescence ReadingsPassive Reference
