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Voorbereiding, toediening en beoordeling van in vivo weefselspecifieke cellulaire opname van fluorescerende kleurstof-gelabelde liposomen
JoVE 신문
생체공학
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JoVE 신문 생체공학
Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes
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Voorbereiding, toediening en beoordeling van in vivo weefselspecifieke cellulaire opname van fluorescerende kleurstof-gelabelde liposomen

3,226 Views

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08:44 min

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July 30, 2020

DOI:

10.3791/61585-v

DOI:
10.3791/61585-v

•

08:44 min
July 30, 2020

•

44 Views
, ,
1Robert M. Berne Cardiovascular Research Center,University of Virginia, 2Department of Pathology,University of Virginia, 3Department of Medicine, Division of Cardiovascular Medicine,University of Virginia

Summary

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Het doel van dit protocol is om fluorescerend gelabelde liposomen te synthetiseren en flowcytometrie te gebruiken om in vivo lokalisatie van liposomen op cellulair niveau te identificeren.

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Cite this Article

Osinski, V., Klibanov, A. L., McNamara, C. A. Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes. J. Vis. Exp. (161), e61585, doi:10.3791/61585 (2020).

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