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Analyse van somatische hypermutatie in de JH4-intron van Germinal Center B-cellen van Mouse Peyer's Patches
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer’s Patches
DOI:

09:35 min

April 20, 2021

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Chapters

  • 00:05Introduction
  • 01:03Dissection of Peyer’s Patches
  • 02:25Cell Isolation and Staining for FACS
  • 05:12JH4 Intron Sequence Amplification and Analysis
  • 07:39Results: Flow Cytometry and JH4 Sequence Analysis
  • 08:52Conclusion

Summary

자동 번역

Hier wordt een test gepresenteerd om somatische hypermutatie binnen de immunoglobuline zware keten gen locus te kwantificeren met behulp van germinale centrum B cellen van muis Peyer's patches.

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