Journal
/
Immunologie und Infektion
/
Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer’s Patches
JoVE Journal
Immunologie und Infektion
This content is Free Access.
JoVE Journal Immunologie und Infektion
Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer’s Patches

Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer’s Patches

6,369 Views

•

09:35 min

•

April 20, 2021

DOI:

10.3791/61551-v

DOI:
10.3791/61551-v

•

09:35 min
April 20, 2021

•

6 Views
, , ,
1Department of Biology,The City College of New York and The Graduate Center of The City University of New York, 2Department of Microbiology,Icahn School of Medicine at Mount Sinai

Summary

Automatically generated

Presented here is an assay to quantify somatic hypermutation within the immunoglobulin heavy chain gene locus using germinal center B cells from mouse Peyer’s patches.

Explore More Videos

Somatic HypermutationJH4 IntronGerminal Center B CellsPeyer’s PatchesAntibody DiversificationMolekularbiologiePCRSanger SequencingCell Isolation

Read Article

Cite this Article

Sible, E., Zheng, S., Choi, J. E., Vuong, B. Q. Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer's Patches. J. Vis. Exp. (170), e61551, doi:10.3791/61551 (2021).

Download .ris file

Copy

Share Video

.

Copy