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小鼠胚胎干细胞中神经祖细胞和神经元的分化与表征
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Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells
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小鼠胚胎干细胞中神经祖细胞和神经元的分化与表征

4,481 Views

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08:47 min

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May 15, 2020

DOI:

10.3791/61446-v

DOI:
10.3791/61446-v

•

08:47 min
May 15, 2020

•

3 Views
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1Departments of Biochemistry and Molecular Biology, Penn State College of Medicine,Penn State Hershey Cancer Institute

Summary

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我们描述了使用悬挂滴法将小鼠胚胎干细胞体外分化为神经元细胞的过程。此外，我们通过RT-qPCR，免疫荧光，RNA-seq和流式细胞术进行全面的表型分析。

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Cite this Article

Hanafiah, A., Geng, Z., Wang, Q., Gao, Z. Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells. J. Vis. Exp. (159), e61446, doi:10.3791/61446 (2020).

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