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Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells
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Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells

Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells

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08:47 min

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May 15, 2020

DOI:

10.3791/61446-v

DOI:
10.3791/61446-v

•

08:47 min
May 15, 2020

•

3 Views
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1Departments of Biochemistry and Molecular Biology, Penn State College of Medicine,Penn State Hershey Cancer Institute

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We describe the procedure for the in vitro differentiation of mouse embryonic stem cells into neuronal cells using the hanging drop method. Furthermore, we perform a comprehensive phenotypic analysis through RT-qPCR, immunofluorescence, RNA-seq, and flow cytometry.

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Mouse Embryonic Stem CellsNeuron DifferentiationNeural ProgenitorsEctodermal DevelopmentHanging Drop CultureEmbryoid BodyNeural Progenitor CellsRetinoic AcidImmunocytochemistry

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Hanafiah, A., Geng, Z., Wang, Q., Gao, Z. Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells. J. Vis. Exp. (159), e61446, doi:10.3791/61446 (2020).

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