Dergi
/
Gelişim Biyolojisi
/
Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells
JoVE Journal
Gelişim Biyolojisi
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Gelişim Biyolojisi
Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells

Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells

DOI:
10.3791/61446-v

•

08:47 min

•

May 15, 2020

•

, , ,
1Departments of Biochemistry and Molecular Biology, Penn State College of Medicine,Penn State Hershey Cancer Institute

Bölümler

  • 00:04Introduction
  • 00:58EB, NPC, and Neuron Differentiation
  • 03:00Fixation and Embedding
  • 04:24Immunofluorescence
  • 05:45Results: E14 Differentiation into EBs, NPCs, and Neurons
  • 07:41Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

We describe the procedure for the in vitro differentiation of mouse embryonic stem cells into neuronal cells using the hanging drop method. Furthermore, we perform a comprehensive phenotypic analysis through RT-qPCR, immunofluorescence, RNA-seq, and flow cytometry.

Etiketler

Mouse Embryonic Stem CellsNeuron DifferentiationNeural ProgenitorsEctodermal DevelopmentHanging Drop CultureEmbryoid BodyNeural Progenitor CellsRetinoic AcidImmunocytochemistry

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Neural Differentiation of Mouse Embryonic Stem Cells in Serum-free Monolayer Culture

Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues

Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells

Efficient Differentiation of Pluripotent Stem Cells to NKX6-1+ Pancreatic Progenitors

Analysis of Retinoic Acid-induced Neural Differentiation of Mouse Embryonic Stem Cells in Two and Three-dimensional Embryoid Bodies

Rapid, Directed Differentiation of Retinal Pigment Epithelial Cells from Human Embryonic or Induced Pluripotent Stem Cells

Isolation, Culture, and Differentiation of Bone Marrow Stromal Cells and Osteoclast Progenitors from Mice

In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells

Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies

Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells

Read Article