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从成人和老化小鼠的河马切片中尽量减少低氧

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Minimizing Hypoxia in Hippocampal Slices from Adult and Aging Mice

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从成人和老化小鼠的河马切片中尽量减少低氧

7,130 Views

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08:58 min

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July 02, 2020

DOI:

10.3791/61377-v

DOI:

10.3791/61377-v

•

08:58 min

July 02, 2020

•

6 Views

Maja Djurisic¹

¹Department of Biology, Neurobiology, and Bio-X,Stanford University

Summary

这是一个急性切片制备从成人和老化的小鼠海马，利用转心灌注和切片切割与冰冷的NMDG-aCSF，以减少对组织的低氧损伤。生成的切片在几个小时内保持健康，并适用于长期的贴片夹和现场记录。

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