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Minimizing Hypoxia in Hippocampal Slices from Adult and Aging Mice
JoVE Journal
Nörobilim
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JoVE Journal Nörobilim
Minimizing Hypoxia in Hippocampal Slices from Adult and Aging Mice

Minimizing Hypoxia in Hippocampal Slices from Adult and Aging Mice

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08:58 min

July 02, 2020

DOI:

08:58 min
July 02, 2020

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Özet

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This is a protocol for acute slice preparation from adult and aging mouse hippocampi that takes advantage of transcardial perfusion and slice cutting with ice-cold NMDG-aCSF to reduce hypoxic damage to the tissue. The resulting slices stay healthy over many hours, and are suitable for long-term patch-clamp and field-recordings.

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