Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Minimizing Hypoxia in Hippocampal Slices from Adult and Aging Mice
7,147 Views
•
08:58 min
•
July 02, 2020
DOI:
Summary
Automatically generated
This is a protocol for acute slice preparation from adult and aging mouse hippocampi that takes advantage of transcardial perfusion and slice cutting with ice-cold NMDG-aCSF to reduce hypoxic damage to the tissue. The resulting slices stay healthy over many hours, and are suitable for long-term patch-clamp and field-recordings.
Read Article
Cite this Article
Djurisic, M. Minimizing Hypoxia in Hippocampal Slices from Adult and Aging Mice. J. Vis. Exp. (161), e61377, doi:10.3791/61377 (2020).
Copy