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Vertering van hele muisogen voor multiparameterstroomcytometrische analyse van mononucleaire fagocyten
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Digestion of Whole Mouse Eyes for Multi-Parameter Flow Cytometric Analysis of Mononuclear Phagocytes
DOI:

09:58 min

June 17, 2020

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Chapters

  • 00:05Introduction
  • 01:13Digestion of Ocular Tissue
  • 03:34Preparation of Cell Suspension
  • 05:19Staining of Cell Suspension for Mononuclear Phagocytes
  • 06:49Results: Mononuclear Phagocyte Populations in the Mouse Eye
  • 09:26Conclusion

Summary

자동 번역

Dit protocol biedt een methode om hele ogen te verteren tot een suspensie met één cel met het oog op cytometrische analyse van de multiparameterstroom om specifieke oculaire mononucleaire fagocytische populaties te identificeren, waaronder monocyten, microglia, macrofagen en dendritische cellen.

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