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全鼠眼的消化，用于单核噬细胞的多参数流细胞学分析
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Digestion of Whole Mouse Eyes for Multi-Parameter Flow Cytometric Analysis of Mononuclear Phagocytes
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全鼠眼的消化，用于单核噬细胞的多参数流细胞学分析

DOI:
10.3791/61348-v

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09:58 min

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June 17, 2020

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, ,
1Department of Ophthalmology, Feinberg School of Medicine,Northwestern University, 2Department of Medicine, Division of Rheumatology, Feinberg School of Medicine,Northwestern University

Chapters

  • 00:05Introduction
  • 01:13Digestion of Ocular Tissue
  • 03:34Preparation of Cell Suspension
  • 05:19Staining of Cell Suspension for Mononuclear Phagocytes
  • 06:49Results: Mononuclear Phagocyte Populations in the Mouse Eye
  • 09:26Conclusion

Summary

자동 번역

자동 번역

该方案提供了一种将整个眼睛消化成单细胞悬浮物的方法，用于多参数流动细胞学分析，以确定特定的眼单核噬细胞群，包括单细胞、微胶质、巨噬细胞和树突细胞。

Tags

DigestionMouse EyesMononuclear PhagocytesFlow CytometryMacrophagesMonocytesMicrogliaDendritic CellsCell Surface MarkersChoroidal NeovascularizationDiabetic RetinopathyExperimental Autoimmune UveitisLupusCytometer ConfigurationDissectionMechanical Disruption

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