Journal
/
신경과학
/
Imaging and Analysis of Neurofilament Transport in Excised Mouse Tibial Nerve
JoVE 신문
신경과학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 신경과학
Imaging and Analysis of Neurofilament Transport in Excised Mouse Tibial Nerve

Imaging and Analysis of Neurofilament Transport in Excised Mouse Tibial Nerve

5,814 Views

•

09:52 min

•

August 31, 2020

DOI:

10.3791/61264-v

DOI:
10.3791/61264-v

•

09:52 min
August 31, 2020

•

3 Views
, , ,
1Department of Neuroscience,The Ohio State University, 2Present address: Interdepartmental Neuroscience Graduate Program and Department of Neurobiology,Northwestern University, 3Quantitative Biology Institute,Ohio University, 4Department of Physics and Astronomy,Ohio University

Summary

Automatically generated

We describe fluorescence photoactivation methods to analyze the axonal transport of neurofilaments in single myelinated axons of peripheral nerves from transgenic mice that express a photoactivatable neurofilament protein.

Explore More Videos

Neurofilament TransportFluorescence MicroscopyPhotoactivatable Fluorescent FusionMyelinated AxonsTibial NerveNerve DissectionPerfusion ChamberOxygenated SalineMicrodissectionNerve Imaging

Read Article

Cite this Article

Boyer, N. P., Azcorra, M., Jung, P., Brown, A. Imaging and Analysis of Neurofilament Transport in Excised Mouse Tibial Nerve. J. Vis. Exp. (162), e61264, doi:10.3791/61264 (2020).

Download .ris file

Copy

Share Video

.

Copy