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Imaging and Analysis of Neurofilament Transport in Excised Mouse Tibial Nerve
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Neurociência
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JoVE Journal Neurociência
Imaging and Analysis of Neurofilament Transport in Excised Mouse Tibial Nerve

Imaging and Analysis of Neurofilament Transport in Excised Mouse Tibial Nerve

5,814 Views

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09:52 min

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August 31, 2020

DOI:

10.3791/61264-v

DOI:
10.3791/61264-v

•

09:52 min
August 31, 2020

•

3 Views
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1Department of Neuroscience,The Ohio State University, 2Present address: Interdepartmental Neuroscience Graduate Program and Department of Neurobiology,Northwestern University, 3Quantitative Biology Institute,Ohio University, 4Department of Physics and Astronomy,Ohio University

Summary

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We describe fluorescence photoactivation methods to analyze the axonal transport of neurofilaments in single myelinated axons of peripheral nerves from transgenic mice that express a photoactivatable neurofilament protein.

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Neurofilament TransportFluorescence MicroscopyPhotoactivatable Fluorescent FusionMyelinated AxonsTibial NerveNerve DissectionPerfusion ChamberOxygenated SalineMicrodissectionNerve Imaging

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Cite this Article

Boyer, N. P., Azcorra, M., Jung, P., Brown, A. Imaging and Analysis of Neurofilament Transport in Excised Mouse Tibial Nerve. J. Vis. Exp. (162), e61264, doi:10.3791/61264 (2020).

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