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Nonradioactive Assay to Measure Polynucleotide Phosphorylation of Small Nucleotide Substrates
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May 08, 2020
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This protocol describes a nonradioactive assay to measure kinase activity of polynucleotide kinases (PNKs) on small DNA and RNA substrates.
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Pillon, M. C., Stanley, R. E. Nonradioactive Assay to Measure Polynucleotide Phosphorylation of Small Nucleotide Substrates. J. Vis. Exp. (159), e61258, doi:10.3791/61258 (2020).
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