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单细胞基因组应用的脂肪组织核分离
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Isolation of Adipose Tissue Nuclei for Single-Cell Genomic Applications
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单细胞基因组应用的脂肪组织核分离

DOI:
10.3791/61230-v

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07:03 min

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June 12, 2020

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,
1Departments of Medicine,Albert Einstein College of Medicine, 2Departments of Molecular Pharmacology,Albert Einstein College of Medicine, 3Fleischer Institute of Diabetes and Metabolism

Chapters

  • 00:04Introduction
  • 01:09Enzymatic Digestion of Adipose Tissue
  • 02:04Adipocyte Isolation
  • 02:59Nuclei Isolation
  • 05:17Results: Sorting and Analysis of Adipocyte Nuclei
  • 06:32Conclusion

Summary

자동 번역

자동 번역

本出版物描述了从成熟的副细胞分离核、通过荧光激活排序和单细胞级转录学进行纯化的协议。

Tags

Single-cell GenomicsNuclei IsolationAdipocytesStromal Vascular FractionCell FiltrationNuclear AggregatesSingle-cell TranscriptomicsAdipose HeterogeneityAdipose KnockoutTransgenic Mice

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