This publication describes a protocol for the isolation of nuclei from mature adipocytes, purification by fluorescence-activated sorting, and single-cell level transcriptomics.
Benitez, G. J., Shinoda, K. Isolation of Adipose Tissue Nuclei for Single-Cell Genomic Applications. J. Vis. Exp. (160), e61230, doi:10.3791/61230 (2020).