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Isolation of Adipose Tissue Nuclei for Single-Cell Genomic Applications
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JoVE Journal Genetica
Isolation of Adipose Tissue Nuclei for Single-Cell Genomic Applications

Isolation of Adipose Tissue Nuclei for Single-Cell Genomic Applications

DOI:
10.3791/61230-v

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07:03 min

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June 12, 2020

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1Departments of Medicine,Albert Einstein College of Medicine, 2Departments of Molecular Pharmacology,Albert Einstein College of Medicine, 3Fleischer Institute of Diabetes and Metabolism

Capitoli

  • 00:04Introduction
  • 01:09Enzymatic Digestion of Adipose Tissue
  • 02:04Adipocyte Isolation
  • 02:59Nuclei Isolation
  • 05:17Results: Sorting and Analysis of Adipocyte Nuclei
  • 06:32Conclusion

Summary

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This publication describes a protocol for the isolation of nuclei from mature adipocytes, purification by fluorescence-activated sorting, and single-cell level transcriptomics.

Tags

Single-cell GenomicsNuclei IsolationAdipocytesStromal Vascular FractionCell FiltrationNuclear AggregatesSingle-cell TranscriptomicsAdipose HeterogeneityAdipose KnockoutTransgenic Mice

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