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Isolation of Adipose Tissue Nuclei for Single-Cell Genomic Applications
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Genetics
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JoVE Journal Genetics
Isolation of Adipose Tissue Nuclei for Single-Cell Genomic Applications

Isolation of Adipose Tissue Nuclei for Single-Cell Genomic Applications

English

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9,589 Views

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07:03 min

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June 12, 2020

DOI:

10.3791/61230-v

DOI:
10.3791/61230-v

•

07:03 min
June 12, 2020

•

11 Views
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1Departments of Medicine,Albert Einstein College of Medicine, 2Departments of Molecular Pharmacology,Albert Einstein College of Medicine, 3Fleischer Institute of Diabetes and Metabolism

Summary

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This publication describes a protocol for the isolation of nuclei from mature adipocytes, purification by fluorescence-activated sorting, and single-cell level transcriptomics.

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Cite this Article

Benitez, G. J., Shinoda, K. Isolation of Adipose Tissue Nuclei for Single-Cell Genomic Applications. J. Vis. Exp. (160), e61230, doi:10.3791/61230 (2020).

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