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A Gnotobiotic System for Studying Microbiome Assembly in the Phyllosphere and in Vegetable Fermentation
Chapters
- 00:04Introduction
- 00:35Growing Germ-free Cabbages
- 03:03Inoculating the Phyllosphere with Microbial Solutions
- 04:56Preparing Sterile Vegetable Extract
- 05:59Results: Growth of Microbial Inoculates in the Napa Cabbage Phyllosphere and Sterile Vegetable Extract
- 07:20Conclusion
A method of growing germ-free Napa cabbages has been developed which enables researchers to evaluate how single microbial species or multispecies microbial communities interact on cabbage leaf surfaces. A sterile vegetable extract is also presented which can be used to measure shifts in community composition during vegetable fermentation.
