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A Gnotobiotic System for Studying Microbiome Assembly in the Phyllosphere and in Vegetable Fermentation
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环境科学
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JoVE 杂志 环境科学
A Gnotobiotic System for Studying Microbiome Assembly in the Phyllosphere and in Vegetable Fermentation

A Gnotobiotic System for Studying Microbiome Assembly in the Phyllosphere and in Vegetable Fermentation

DOI:
10.3791/61149-v

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07:51 min

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June 03, 2020

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, , ,
1Department of Biology,Tufts University

Chapters

  • 00:04Introduction
  • 00:35Growing Germ-free Cabbages
  • 03:03Inoculating the Phyllosphere with Microbial Solutions
  • 04:56Preparing Sterile Vegetable Extract
  • 05:59Results: Growth of Microbial Inoculates in the Napa Cabbage Phyllosphere and Sterile Vegetable Extract
  • 07:20Conclusion

Summary

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A method of growing germ-free Napa cabbages has been developed which enables researchers to evaluate how single microbial species or multispecies microbial communities interact on cabbage leaf surfaces. A sterile vegetable extract is also presented which can be used to measure shifts in community composition during vegetable fermentation.

Tags

Keywords: Gnotobiotic SystemMicrobiome AssemblyPhyllosphereVegetable FermentationSterile Vegetable ExtractPhyllo Sphere CommunitiesSurface SterilizeCabbage SeedsMS BrothSterile Cabbage SeedGerm-free CabbageSterility TestCabbage Homogenate

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